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Synthesis of RNA Bearing C5-polyamine Modified Pyrimidine Nucleosides at the 3´-end by T4 RNA Ligase

[ Vol. 16 , Issue. 2 ]

Author(s):

Tomokazu Masuda, Shingo Kimura, Mohammad Mehedi Masud and Hiroaki Ozaki*   Pages 122 - 127 ( 6 )

Abstract:


The incorporation of the modified nucleoside into the 3´-overhang regions of short interfering RNAs (siRNAs) has been reported to enhance their nuclease resistance and improve RNA interference activity. In this study, DNA 2-mers containing C5-polyamine-modified pyrimidine nucleosides were synthesized and then ligated to the 3´-end of the RNA by T4 RNA ligase. The modification of the base moiety with tris(2-aminoethyl)amine affected the ligation efficiency, but the DNA 2-mer containing only one modified nucleoside was ligated with sufficient efficiency. We propose a novel synthetic route to modified siRNA bearing various modified groups in the 3´-overhang region.

Keywords:

C5-modified pyrimidine, siRNA, T4 RNA Ligase, tris(2-aminoethyl)amine, oligonucleotides, genes.

Affiliation:

Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 4-2 Aramaki, Maebashi, Gunma 371-8510, Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 4-2 Aramaki, Maebashi, Gunma 371-8510, Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 4-2 Aramaki, Maebashi, Gunma 371-8510, Division of Pure and Applied Science, Faculty of Science and Technology, Gunma University, 4-2 Aramaki, Maebashi, Gunma 371-8510

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