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Improved Scalable Synthesis and Biological Activity of “Indoxyl-gal,” a Chromogenic Histochemical Used for the Identification of Lac+ Organisms

[ Vol. 15 , Issue. 10 ]

Author(s):

Brent Banasik*, Tam Mai and Mansour Samadpour   Pages 822 - 825 ( 4 )

Abstract:


An improved and operationally simple synthesis of 3-indoxyl-β-D-galactopyranoside (indoxyl- gal) is disclosed. Indoxyl-gal is a sensitive chromogenic histochemical used for the identification of β-galactosidase enzymatic activity (lacZ gene). Synthesis of indoxyl-gal is improved to 25% overall yield in 5 linear steps from affordable starting materials with minimal chromatography. Biological testing confirmed the detection of β-galactosidase activity in all (100%, 19 strains) coliform bacteria using membrane filtration and modified MI agar.

Keywords:

3-Indoxyl-β-D-galactopyranoside, β-galactosidase, chromogenic dye, coliform bacteria, histochemistry, indicators.

Affiliation:

Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155, Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155, Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155

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