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The Novel Synthesized Pyridazinone Derivates had the Antiproliferative and Apoptotic Effects in SHSY5Y and HEP3B Cancer Cell Line

[ Vol. 15 , Issue. 4 ]

Author(s):

Osman Ciftci*, Zeynep Ozdemir, Ceren Acar, Mert Sozen, Nese Basak-Turkmen, Idris Ayhan and Harika Gozukara   Pages 323 - 331 ( 9 )

Abstract:


Background: Brain cancer (neuroblastoma) and liver cancer (hepatocellular carcinoma) are common cancer types among others worldwide which do not have a radical treatment and cure.

Objective: In the current study, five novel pyridazinone derivates bearing benzelhydrazone moiety at second position were synthesized and evaluated for their cytotoxic activity against neuroblastoma and hepatocellular carcinoma (SHSY5Y and HEP3B) and human fibroblast (HF) cell lines. The aim of the current study is to identify antiproliferative activity of five novel pyridazinone derivates against neuroblastoma and hepatocellular carcinoma (SHSY5Y and HEP3B) and human fibroblast (HF) cell lines.

Method: The compounds were synthesized by the reacting 6-[4-(phenyl/4-chlorophenyl)piperazine-1- yl]-3(2H)-pyridazinone-2-yl acetohydrazide with benzaldehyde in ethanol. The in vitro antiproliferative activities were determined with MTT assay. Bax, Bcl-2 and Casp3 gene expression levels were detected with RT-PCR analyses.

Results: The lowest IC50 was observed for compound 4 in SHSY5Y and HEP3B cells. Apoptosis increased in cancer cells which was shown by changes inBax, Bcl-2 and Casp3 gene expression levels with 1-5 compound therapy.

Conclusion: Novel pyridazinone derivates might be promising agents as new chemotherapeutic candidates in brain and liver cancer.

Keywords:

Apoptotic effects, antitumoral effects, brain cancer, cytotoxic effects, liver cancer, pyridazinone.

Affiliation:

Department of Medical Pharmacology, Faculty of Medicine, Inonu University, 44280 Malatya, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Inonu University, 44280 Malatya, Department of Molecular Biology and Genetics, Faculty of Art and Science, Inonu University, 44280 Malatya, Department of Molecular Biology and Genetics, Faculty of Art and Science, Inonu University, 44280 Malatya, Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Inonu University, 44280 Malatya, Department of Medical Pharmacology, Faculty of Medicine, Inonu University, 44280 Malatya, Department of Biostatistics, Faculty of Medicine, Inonu University, 44280 Malatya

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